Detection of Chlamydia trachomatis in endocervical smears of sexually active women in Manaus-AM, Brazil, by PCR.
نویسندگان
چکیده
Chlamydia trachomatis is now one of the most prevalent bacteria found in classic sexually transmissible diseases (STD), and as such, constitutes a serious public health problem. We examined the prevalence of Chlamydia trachomatis, by polymerase chain reaction (PCR), in 121 sexually active women who sought treatment for STD in the Alfredo da Matta Institute of Dermatology and Venerology and the Institute of Tropical Medicine of Amazonas in Manaus, Brazil. These women were examined by a specific PCR for the chlamydial plasmid, and the nature of the amplicon was determined by restriction analysis and DNA sequencing. The PCR diagnosis revealed a prevalence of 20.7% infected women.
منابع مشابه
The Prevalence of Chlamydia trachomatis Infection in Women Attending Health Clinics in Shiraz, Islamic Republic of Iran
Background Chlamydia trachomatis (CT) is a common cause of sexually transmitted infections (STI) and a prevalent microorganism found in the vaginal discharge of sexually active women. The infection usually has no symptoms; although it may cause chronic complications such as pelvic inflammatory disease (PID), ectopic pregnancy, chronic pelvic pain, tubal infertility and cervical cancer. This stu...
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Background Chlamydia trachomatis infections are the most prevalent sexually transmitted bacterial infections (STI) in the world that lead to a cause of tubal factor infertility in women. The aim of this study is to determine the presence of C.trachomatis by polymerase chain reaction (PCR) and ELISA. MaterialsAndMethods Endocervical swabs were collected from 80 women; 22 of them were asymptomati...
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متن کاملGenotyping of Chlamydia trachomatis from endocervical specimens in Shiraz, Iran
The objective of this study was to determine the prevalence and distribution of Chlamydia trachomatisgenotypes in Shiraz, Iran. Two hundred twelve cervical swab samples were collected from women attendingShahid Motahari Polyclinic in Shiraz, Iran. The endocervical specimens were screened for C. trachomatis byplasmid PCR. Genotyping was performed in C. trachomatis-positive samples by nested PCR ...
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عنوان ژورنال:
- The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases
دوره 7 2 شماره
صفحات -
تاریخ انتشار 2003